Combustion particle enhanced nasal allergic response: a human experimental exposure study.
Hauser-R; Rice-T; Krishna-Murthy-GG; Wand-MP; Siegel-P; Paulauskis-J
Am J Respir Crit Care Med 2001 Apr; 163(5)(2)(Suppl):A159
We conducted a human experimental exposure study to investigate the interaction of particles and allergens. Volunteers with and without atopy were exposed to combustion particles and then challenged with an aeroallergen (determined by skin testing). The particles and allergens were delivered nasally. The whole pollen grains were delivered using a vibrating drum apparatus. The particulates, fuel oil ash, were delivered using a Wright dust feeder. The mean (SO) particle exposure concentration was 1.02 (0.23) mg/m3 for one-hour. We tested six subjects, three atopic subjects and three without atopy. Each subject participated in three randomized exposure-challenge sessions at least 2 weeks apart (i.e. clean air followed by allergen, particles followed by no allergen, particles followed by allergen). The exposure/challenge sessions occurred out-of-allergy season. Nasal lavage (NL) was performed immediately prior to particle/clean air- exposure, and immediately after challenge, and 4, 18, 42 and 66 hours post challenge. Cell counts and differentials were performed on each NL. Total cells/mL and neutrophils/mL increased by 30 x 103 (p=0.06) and 28x103 cells/mL (p=0.06), respectively when allergen challenge was preceded by particle exposure as compared to when it was preceded by clean air exposure. We found evidence of an interaction between particulate exposure and allergen challenge. Analysis of nasal lavage cytokine data is ongoing.
Respiratory-irritants; Respiratory-system-disorders; Respirable-dust; Particulate-dust; Dust-particles; Dust-exposure; Dust-inhalation; Airborne-dusts; Allergens; Allergic-reactions; Allergies; Nasal-cavity
American Journal of Respiratory and Critical Care Medicine. Abstracts of the American Thoracic Society 2001 International Conference, May 18-23, 2001, San Francisco, California