Hair as a target tissue for protein-bound pyrroles following subchronic intra peritoneal injections of 2,5-hexanedione.
Johnson-JD; Lack-L; Abdel-Rahman-SM; Abou-Donia-MB
Toxicologist 1994 Mar; 14(1):360
Studies were carried out to ascertain the feasible use of body hair as a biological marker for chronic exposure to industrial neurotoxicants that yield the metabolite, 2,5-hexanedione (2,5-HD), i.e., n-hexane and methyl-n-butyl ketone. 2,5-HD is capable of forming N-substituted pyrroles by reacting with primary amines which include amino acids as well as the E-amino groups of lysine-containing peptides. Male SpragueDawley rats were given daily ip. injections of 50 mg/kg 2,5-HD for 45 days. At intervals, hair samples distant from the site of injection of treated animals were taken and showed staining which was positive for the presence of pyrroles with Ehrlich's reagent (p-N,N-dimethylaminobenzaldehyde), whereas control animals were negative. In addition, proteins were solubilized from these samples. The protein solutions from the treated samples tested positive for the presence of pyrroles with Ehrlich's reagent and upon spectral analysis yielded an absorbance peak of 530 nm which is in the range characteristic of this type of pyrrole. The extent of positive staining for pyrroles in the vibrissae progressed linearly with time. These findings suggest the potential use of hair as an indicator for chronic exposure to this class of potential industrial neurotoxic chemicals and as a complement to the urinary analysis which is used to confirm recent exposure.
Laboratory-testing; Toxins; Toxicology; Toxic-effects; Exposure-assessment; Exposure-levels; Central-nervous-system; Chronic-exposure; Chronic-toxicity; Neurotoxic-effects; Neurotoxins; Animal-studies; Laboratory-animals; Pyrroles
The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
Duke University Medical Center, Durham, North Carolina