The effect of Fe2O3 and AI2O3 on the metabolism of benzo(a)pyrene by pulmonary alveolar macrophage.
Toxicologist 1994 Mar; 14(1):339
The respiratory tract is a common route for entry of toxic materials from modern urban and working environments. Fe203 and AI2O3 particles and benzo(a)pyrene (BaP) are widely encountered in the occupational settings. The aim of this project is to study the role of particles on the modulation of metabolism of BaP. AM from male-Syrian Golden hamsters were incubated in a plate assay with BaP(5 ug) alone or BaP (5 ug)-coated respirable size Fe203 and Al203 from doses of 0.5 mg to 2.0 mg. After 24 hr the metabolic profiles indicate that the release of diol, phenol, quinone and total BaP metabolites was significantly greater with Fe2O3 compared to AI2O3 or BaP alone. The formation of 7,8-diol-BaP, the procarcinogen, was also consistent with the increase of Fe2O3 doses. Coadministration of 7,8-benzoflavone, an inhibitor of cytochrome P-450s, significantly reduced the BaP metabolism in BaP-coated Fe2O3 and AI2O3 treatments. These data suggest that particles enhance the metabolism of BaP in AM via the modulation of P-450 isozymes.
Laboratory-testing; Toxins; Toxicology; Toxic-effects; Exposure-assessment; Exposure-levels; Laboratory-animals; Animal-studies; Iron-oxides; Pyrenes
1345-25-1; 1344-28-1; 50-32-8
The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
Univesity of Cincinnati, Cincinnati, Ohio