Immunohistochemical characterization of carbon disulfide (CS2) induced cytoskeletal alterations in the rat central nervous system.
Jensen-KF; Wilmarth-KR; Olin-JK; Abou-Donia-MB
Toxicologist 1994 Mar; 14(1):205
Male Sprague-Dawley rats were exposed to 200 ppm concentrations of CS2 via inhalation for 10h/day over 140 consecutive days. Control animals were housed in inhalation chambers and supplied with ambient air for the duration of the study. Animals were perfused 24h following the1ast exposure, with 4% paraformaldehyde in 0.1 M acetate buffer. Brains and spinal cords were removed and processed for immunohistochemistry. Sections were stained with monoclonal antisera against phosphorylated and nonphosphorylated neurofilaments (SMI-3I, SMI-33, Sternberger Monoclonals Inc.) or vimentin (V9, Boehringer Mannheim). Enlarged axons were apparent in the spinal cord and brainstem of treated animals. These abnormal axons were not restricted to regions containing the largest axons indicating that this exposure schedule may alter smaller axons as well as larger ones. In treated animals, neurofilament aggregations could be demonstrated with either SMI-31 or SMI-33. The aggregations appeared to differ in the relative amounts of phosphorylated and nonphosphorylated neurofilaments, suggesting the concomitant presence of various states of axonal degeneration and regeneration. The antisera against vimentin faintly labeled the region around the central canal of the spinal cord in control animals. In contrast, distinctive staining was consistently observed in the ventral columns, the tip of the dorsal horn, and the central canal in treated animals. We are currently investigating the cellular localization of CS2 induced vimentin-like staining.
Laboratory-testing; Toxins; Toxicology; Toxic-effects; Exposure-assessment; Exposure-levels; Laboratory-animals; Animal-studies; Central-nervous-system; Histochemical-analysis; Immunological-tests; Immunotoxins
The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
Duke University Medical Center, Durham, North Carolina