Endotoxin and grain dust-induced lung inflammation.
Schwartz-DA; Jagielo-PJ; Bleuer-SA; Frees-K
Toxicologist 1994 Mar; 14(1):88
Dose-response relationships were studied for inhaled grain dust extracts and endotoxin with bronchoalveolar lavage fluid (BALF) concentrations of total cells, neutrophils, and tumor necrosis factor-a (fNF). The inhalation model utilized C3H/HeJ mice that are unresponsive to endotoxin and C3H/HeBFFJ endotoxin responsive mice. Exposures were performed in groups of 12 (6 of each strain) for 4 hr. 20 groups of mice were exposed to aerosols of 0.1 % corn dust extract (CDE), filter sterilized CDE (SCDE), E. coli endotoxin (LPS), or saline. After exposure, lungs were lavaged and fixed for histological study. Endotoxin resistant mice were unresponsive to LPS and demonstrated only minor responses to CDE and SCDE at high doses. Endotoxin sensitive mice demonstrated significantly greater (p < 0.001) dose-dependent responses for CDE, SCDE, and LPS than the endotoxin resistant mice over a SOO-fold dose range. There were no significant within-strain differences for neutrophil and TNF-a responses upon exposure to these agents. Further, the endotoxin resistant mice demonstrated a blunted inflammatory response to both CDE and SCDE. These findings suggest that endotoxin may be a key agent in acute grain dust-induced lung inflammation.
Lung-fibrosis; Lung-function; Mathematical-models; Statistical-analysis; Farmers; Dose-response; Laboratory-animals; Endotoxins
Internal Medicine University of Iowa Pulmonary Disease Division Iowa City, IA 52242
Grant; Cooperative Agreement
Pulmonary System Disorders
The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
University of Iowa, Iowa City, Iowa