Overexpression of the erbB-2 proto-oncogene in canine osteosarcoma cell lines and tumors.
Flint-AF; U'Ren-L; Legare-ME; Withrow-SJ; Dernell-W; Hanneman-WH
Vet Pathol 2004 May; 41(3):291-296
The status of the erbB-2 (human epidermal growth factor receptor 2/neu) proto-oncogene in canine osteosarcoma (OSA) has not been reported previously. In this study we used real-time reverse transcriptase polymerase chain reaction to evaluate erbB-2 expression in seven canine OSA cell lines and 10 canine OSA tissue samples. We determined erbB-2 to be significantly overexpressed in 86% (six of seven) of the cell lines and 40% (4 of 10) of the OSA tissues samples. Given the importance of erbB-2 in human breast cancer, the finding of erbB-2 overexpression in canine OSA may be important in further understanding the pathogenesis and possible therapies of OSA.
Animal-studies; Animals; Chemical-reactions; Chemical-synthesis; Cancer; Cancer-rates; Pathogenesis; Pathogenicity; Pathogens; Growth-disorders; Growth-factors; Growth-inhibition; Growth-inhibitors; Oncogenesis; Oncogenic-agents; Oncogenicity; Bone-cancer; Bone-disorders; Bone-structure; Exposure-levels; Exposure-limits; Exposure-methods;
Author Keywords: Canine model; dogs; erbB-2; HER2; osteosarcoma
A. F. Flint, Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Campus Delivery, Building Physiology 1680, Fort Collins, CO 80523
Agriculture; Cooperative Agreement
Colorado State University - Fort Collins