Comparative proteomics, genomics and pulmonary toxicity of instilled single walled carbon nanotubes, crocidolite asbestos and ultrafine carbon black in mice.
Teeguarden-J; Waters-K; Webb-Robertson-B; Varnum-S; Jacobs-J; Zangar-R; Kisin-E; Murray-A; Shvedova-A; Pounds-J
Toxicologist 2009 Mar; 108(1):460
CNT have emerged as a nanomaterial of considerable concern to the occupational and environmental health and safety communities. Early, consistent reports from high-dose, short-term screening studies in rodents suggest a sequence of biological events and pulmonary pathology similar to that caused by asbestos and synthetic vitreous fibers (SVF). Whether CNT strictly adhere to the asbestos paradigm of pulmonary toxicity is a question of critical importance. The pulmonary response of mice exposed repeatedly (4 doses, 28 days, pharyngeal aspiration) to single-walled CNT (SWCNT), crocidolite asbestos (AS) and ultrafine carbon black (CB) was assessed by global proteomics and genomics of lung tissue and ELISA protein microarray of bronchial lavage fluid. Three cytokines, IL-6 and IL-12 and Mip-1g were considerably higher in SWCNT and AS treated animals compared to CB. The T cell chemotactic protein MDC was present in high levels in SWCNT and AS groups, but not detectable in control or CB treated groups. Two sentences on similarities and differences via proteomics. Microarray analysis of lung tissue revealed over 3000 genes significantly changed by SWCNT exposure, compared to only 469 with AS and 71 with CB. Strikingly, the significant genes lists for CB and AS were complete subsets of the SWCNT data set, with both the number of genes and magnitude of gene changes being the highest in the SWCNT treatment group.
Biological-effects; Biological-factors; Cellular-reactions; Cell-biology; Cytology; Exposure-assessment; Exposure-levels; Exposure-methods; Environmental-exposure; Environmental-hazards; Fibrogenicity; Fibrosis; Fibrous-bodies; Fibrous-dusts; Genotoxic-effects; Health-hazards; Irritants; Inhalation-studies; Laboratory-animals; Lung-cells; Lung-disorders; Lung-irritants; Microscopic-analysis; Microscopy; Oxidative-metabolism; Oxidative-processes; Particle-aerodynamics; Particulates; Pulmonary-disorders; Pulmonary-system-disorders; Respiratory-irritants; Respiratory-system-disorders; Statistical-analysis; Toxic-effects; Nanotechnology
7440-44-0; 12001-28-4; 1332-21-4; 1333-86-4
The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland