Biodegradation of single walled carbon nanotubes through peroxidase catalysis.
Konduru-NV; Vlasova-II; Allen-BL; Kisin-ER; Murray-AR; Kapralov-AA; Star-A; Shvedova-AA; Kagan-VE
Toxicologist 2009 Mar; 108(1):458
Management of exposure to bioengineered single walled carbon nanotubes (SWCNT) is becoming a major environmental and health challenge. Because of unique characteristics, SWCNT have found increased applications in various fields of science and technology. With increased risk of human and environmental exposure, strategies to degrade SWCNT are also garnering interest. Here, we show - for the first time - that two peroxidases, namely myeloperoxidase and horseradish peroxidase are effective in catalyzing the biodegradation of SWCNT. A systematic characterization of resulting products of degradation was performed using transmission electron microscopy (TEM), dynamic light scattering (DLS), gel electrophoresis, mass spectrometry, UV-Vis-NIR spectroscopy and thermogravimetric analysis (TGA). We also demonstrate that unlike na´ve non-biodegraded nanoparticles, the peroxidase degraded particles did not elicit pulmonary inflammatory response in mice as evidenced by the release of pro-inflammatory cytokines and the content of neutrophils (PMN) in bronchoalveolar lavage. These results mark a novel approach to employ peroxidase catalysis for directed biodegradation of carbon nanotubes in biofluids/tissues as well as in environmental settings.
Biological-effects; Biological-factors; Cellular-reactions; Cell-biology; Cytology; Environmental-exposure; Environmental-hazards; Environmental-health; Exposure-assessment; Exposure-levels; Exposure-methods; Health-hazards; Irritants; Inhalation-studies; Laboratory-animals; Lung-cells; Lung-disorders; Lung-irritants; Microscopic-analysis; Microscopy; Oxidative-processes; Particle-aerodynamics; Particulate-dust; Particulates; Respiratory-irritants; Respiratory-system-disorders; Risk-factors; Nanotechnology
The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland
University of Pittsburgh at Pittsburgh