Identification of indoor air contaminates using an in vitro exposure system.
Jackson-L; Wells-R; Munson-AE; Anderson-SE
Toxicologist 2009 Mar; 108(1):313
On average, U.S. citizens spend 80% or more of their daily lives indoors whether at home, work, or in other commercial buildings. Over the last two decades, there has been increasing awareness regarding the potential impact of indoor air pollution on health. These studies use an in vitro monitoring system called the VitroCell which utilizes the air/cell interface allowing for direct contact between cells and componentsof a test atmosphere to assess chemicals found in the indoor air environment. The structurally similar dicarbonyls diacetyl, 4-oxopentanal, gluteraldehyde and methylglyoxal were selected for use in this system. Exposure to these compounds, which are found in the indoor environment, has been suggested to contribute to adverse health effects. The VitroCell module was used to evaluate immune-related gene and protein expression generated after a non-immune (pulmonary epithelial A549) and immune (alveolar macrophage) cell line were exposed to these aerosolized chemicals. A low density real time PCR gene array, screening 84 immune- related genes, was used to investigate the exposure effects of these compounds. Alterations in the inflammatory cytokines IL-8 and TNF-alpha were identified after exposure to these compounds. The identified cytokines can potentially be used as biomarkers to screen contaminated indoor air environments. These studies may provide an in vitro method for the identification and characterization of chemical hazards including indoor air pollutants in work environments such as office buildings, allowing for the reduction of worker illness and more specifically reducing respiratory consequences of exposures to allergens and irritants.
Air-quality-measurement; Air-sampling; Aerosols; Air-monitoring; Air-purification; Air-quality; Air-quality-monitoring; Air-contamination; Biological-factors; Biomarkers; Biological-monitoring; Biological-effects; Chemical-hypersensitivity; Employee-exposure; Exposure-assessment; Exposure-methods; Genes; Genetic-factors; Inhalation-studies; Indoor-environmental-quality; Immune-system; Immunodiagnosis; Immunological-tests; Immunology; Occupational-exposure; Occupational-hazards; Occupational-health; Pulmonary-system; Respiratory-hypersensitivity; Respiratory-system-disorders; Respiratory-hypersensitivity; Respiratory-irritants; Work-environment; Workplace-studies
Healthcare and Social Assistance; Services
The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland