The production and characterization of monoclonal antibodies against toluene diisocyanate (TDI)-conjugated proteins.
Schmechel-D; Ruwona-TB; Hettick-JM; Janotka-E; Blachere-FM; Beezhold-DH; Siegel-PD
J Allergy Clin Immunol 2009 Feb; 123(2)(Suppl 1):S150
RATIONALE: Toluene diisocyanate (TDI) is a highly reactive chemical and one of the most commonly reported causes of occupational asthma. The production of TDI-specific monoclonal antibodies (mAb) will allow the development of standardized immunoassays for exposure assessment. METHODS: Mice were immunized with TDI-keyhole limpet hemocyanin (KLH) conjugates and hybridomas were screened by ELISA using TDI conjugated to human serum albumin. MAbs were characterized by western blot against various mono- and diisocyanates conjugated to different carrier proteins using carbamate or thiocarbamate linkages. RESULTS: A total of 35 mAbs were produced (25 IgG1, 8 IgG2a, 2 IgG2b). Several mAbs were found to be specific for 2,4-TDI-conjugated proteins and did not react with 2,6-TDI-, monoisocyanate- or isothiocyanate -conjugated proteins. Other mAbs cross-reacted with the above conjugates, methylene diphenyldiisocyanate- and hexamethylene diisocyanate protein conjugates. All mAb reactivities were carrier protein independent. CONCLUSIONS: The mAbs can differentiate between specific isocyanates in a carrier independent fashion and may be useful as hapten-specific reagents for immunoassay development.
Antigens; Antibody-response; Biochemical-analysis; Biochemistry; Biological-effects; Biological-factors; Biological-systems; Breathing; Immune-reaction; Immunochemistry; Humans; Laboratory-animals; Laboratory-testing
Healthcare and Social Assistance; Services
Journal of Allergy and Clinical Immunology