Phosphatidylserine targets single-walled carbon nanotubes to professional phagocytes in vitro and in vivo.
Konduru-NV; Tyurina-YY; Feng-W; Basova-LV; Belikova-NA; Bayir-H; Clark-K; Rubin-M; Stolz-D; Vallhov-H; Scheynius-A; Witasp-E; Fadeel-B; Kichambare-PD; Star-A; Kisin-ER; Murray-AR; Shvedova-AA; Kagan-VE
PLoS One 2009 Feb; 4(2):e4398
Broad applications of single-walled carbon nanotubes (SWCNT) dictate the necessity to better understand their health effects. Poor recognition of non-functionalized SWCNT by phagocytes is prohibitive towards controlling their biological action. We report that SWCNT coating with a phospholipid ''eat-me'' signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. Macrophage uptake of PS-coated nanotubes was suppressed by the PS-binding protein, Annexin V, and endocytosis inhibitors, and changed the pattern of pro- and anti-inflammatory cytokine secretion. Loading of PS-coated SWCNT with pro-apoptotic cargo (cytochrome c) allowed for the targeted killing of RAW264.7 macrophages. In vivo aspiration of PS-coated SWCNT stimulated their uptake by lung alveolar macrophages in mice. Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells.
Inhalation-studies; Particulates; Airborne-particles; Airborne-dusts; Air-treatment; Pulmonary-function-tests; Lung-irritants; Pulmonary-system-disorders; Laboratory-animals; Laboratory-testing; Biological-effects; Biological-monitoring; Biological-transport; Cell-biology; Cell-function; Cellular-function; Cellular-reactions; Nanotechnology
Public Library of Science One
University of Pittsburgh at Pittsburgh