Entrapment of ionic tris(2,2'-bipyridyl) ruthenium(II) in hydrophobic siliceous zeolite: O2 sensing in biological environments.
Ruda-Eberenz TA; Nagy A; Waldman WJ; Dutta PK
Langmuir 2008 Aug; 24(16):9140-9147
Synthesis of the ionic dye, tris(2,2'-bipyridyl) ruthenium(II) chloride (Ru(bpy)32+·2Cl-) within the supercages of a highly hydrophobic zeolite Y is reported. Use of the neutral precursor Ru(bpy)Cl2(CO)2 allowed for high loading levels of Ru(bpy)32+ (1 per 7 and 25 supercages). The emission quenching of the Ru(bpy)32+-zeolite crystals dispersed in polydimethoxysiloxane (PDMS) films by dissolved oxygen in water was examined. The quenching data as a function of oxygen concentration was fit to a linear Stern-Volmer plot (R2 = 0.98). Using the Stern-Volmer plot as calibration, changes in concentration of dissolved oxygen due to reaction with glucose in the presence of glucose oxidase was monitored. Human monocyte-derived macrophages internalized the submicron-sized Ru(bpy)32+-zeolite crystals, and intracellular oxygen concentrations initiated by zymosan-mediated oxidative burst could be monitored by measuring the emission from Ru(bpy)32+ by confocal fluorescence microscopy.
Particulates; Particulate-dust; Metallic-compounds; Oxygen-transport; Biological-factors; Biological-systems
Prabir K. Dutta, Department of Pathology, The Ohio State University College of Medicine, 4160 Graves Hall, 333 West 10th Avenue, Columbus, OH 43210
1318-02-1; 7782-44-7; 50-99-7
The Ohio State University