Detection of polycyclic aromatic hydrocarbon-DNA adducts in placental DNA samples by room-temperature solid-matrix phosphorescence.
Hurtubise-RJ; Thompson-AL; Weston-A; Manchester-DK
Anal Lett 2008 Jan; 41(11):1944-1963
This is the first attempt for the direct detection of polycyclic aromatic hydrocarbon (PAH)-DNA adducts in human placental DNA samples by solid-matrix phosphorescence (SMP). Six samples were investigated, and SMP emission spectra and the corresponding second derivative SMP spectra were obtained for all the samples. Numerous excitation and emission wavelengths were studied for detecting PAH-DNA adducts. Second derivative SMP spectra indicated the presence of PAH-DNA adducts, whereas the longer SMP emission region proved fruitful for detecting adducts in the placental DNA samples. The SMP results for the samples strongly implied that a variety of PAH-DNA adducts could be present.
Polycyclic-aromatic-hydrocarbons; Polycyclic-hydrocarbons; Phosphorous-compounds; DNA-adducts; Sample-preparation; Sampling-methods
Robert J. Hurtubise, University of Wyoming, Department of Chemistry, Laramie, WY 82071