The effect of lecithin surfactant and phospholipase enzyme treatment on some cytotoxic properties of respirable quartz and kaolin dusts.
Wallace-WE; Keane-BMJ; Hill-CA; Vallyathan-V; Saus-F; Castranova-V; Bates-D
Proceedings of the International Symposium on Respirable Dust in the Mineral Industries: Health Effects, Characterization, and Control, University Park, Pennsylvania, October 14-16, 1986. University Park, PA: Pennsylvania State University, 1988; :154-166
Two in vitro systems are being developed to model initial stages in the interaction of respired dusts with the acinar region of the lung. Incubation of quartz and kaolin with dipalmitoyl glyceropholphorylcholine, a major component of pulmonary surfactant, suppresses the prompt cytotoxicity of both dusts as measured by in vitro enzyme disease from pulmonary macrophages or assay by erythrocyte hemolysis. Additional data is presented to quantitate the detoxification or quartz and kaolin by dipalmitoyl lecithin. Subsequent incubation of lecithin treated dusts with phospholipase enzymes results in partial to complete restoration of the assayed cytotoxic potentials, dependent on the lipase activity applied, the incubation time, and the mineral. Restored cytotoxic potential is due to removal of the prophylactic lecithin surface coating and/or due to residual lysolecithin which is produced by the partial enzymatic digestion of lecithin. Data is presented on the contribution of each mechanism to the restored toxicity. Again, this is dependent on the mineral used, the lipase activity applied, and digestion time. Additionally, a system is under development to challenge pulmonary macrophages in culture with native and surfactant treated dusts. A preliminary test of the dipalmitoyl lecithin - quartz - rabbit pulmonary macrophage system is presented to illustrate the method.
Respirable-dust; Mineral-dusts; Quartz-dust; Silica-dusts; Lung-irritants; Alveolar-cells; Surfactants; Toxic-materials; Dust-inhalation; Dust-particles; Respiratory-irritants; Cytotoxins; Cytotoxic-effects
Proceedings of the International Symposium on Respirable Dust in the Mineral Industries: Health Effects, Characterization, and Control, University Park, Pennsylvania, October 14-16, 1986