Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting.
Hettick-JM; Green-BJ; Buskirk-AD; Kashon-ML; Slaven-JE; Janotka-E; Blachere-FM; Schmechel-D; Beezhold-DH
Rapid Commun Mass Spectrom 2008 Aug; 22(16):2555-2560
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5,000-20,000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13,900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance.
Fungi; Spectrographic-analysis; Spectroscopes; Statistical-analysis; Biomarkers; Clinical-chemistry; Clinical-tests; Environmental-factors; Environmental-medicine; Mass-spectrometry; Microbial-test-systems; Microorganisms; Microscopic-analysis; Diagnostic-tests
Justin M. Hettick, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, ACIB, MS L-2040, 1095 Willowdale Road, Morgantown, WV 26505-2888
Rapid Communications in Mass Spectrometry