Because of the high occupant density in aircraft, the surface chemistry of ozone and squalene, an important component of skin oil, was evaluated. A reaction probability of (45+/-14)×10^-5 was determined for the reaction of squalene (2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene) with ozone (50 parts per billion (ppb)) on a glass plate surface using the Field and Laboratory Emission Cell (FLEC) Automation and Control System (FACS). To more clearly define part of squalene's indoor environment degradation mechanism, gas-phase and surface-bound products of the squalene+O3 reaction were also investigated. Emitted products were captured in solution, derivatized with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA), and analyzed by gas chromatography and ion trap mass spectroscopy. The identified squalene+O3 reaction products were: 6-methyl-5-hepten-2-one (6MHO, ((CH3)2C=CH(CH2)2 C(=O)CH3)), glyoxal (ethanedial, HC(=O)C(=O)H), 4-oxopentanal (4OPA, CH3C(=O)CH2CH2 CH(=O)), and 6,10-dimethylundeca-5,9-dien-2-one (geranyl acetone). The compound 5,9,13-trimethyltetradeca-4,8,12-trienal is proposed as the other major squalene+O3 reaction product. This compound was determined from mass spectrometry coupled with plausible squalene+O3 reaction mechanisms based on previously published volatile organic compound+O3 gas-phase reaction mechanisms.