The effect of cobalt, tungsten, tungsten-cobalt, and cobalt chloride on L-selectin expression in K562, J-11, and Daudi cells and on ICAM-1 expression in L2 cells.
Mol Biol Cell 1997 Nov; 8(Suppl):1689
We studied the effect of cobalt. tungsten and tungsten/cobalt particles on the expression of L-selectin at the protein level in a cell line (K562) transfected with a cDNA clone of L-selectin and in two lymphoid cell lines which express L-selectin constitutively (Daudi and J-11). Cell surface receptor expression was determined by flow cytometry. The cell lines were incubated with 5IJg/mi of the metal par1\cles for 24, 48 and 72 hours. There was no difference in L-selectin expression in control and experimental samples. We also studied the effect of these metals on the expression of ICAM-1 in a rat lung epithelial-like cell line (L-2, ATCC) at the mRNA level. mRNA levels were measured by semi- quantitative RT -PCR. ICAM-1 mRNA was analyzed in L2 ceils after 24h Incubation with metal particles at 1 ug/ml, 3 ug/mi. 5 ug/mi. and 7 ug/ml No significant differences were found in the ICAM-1 mRNA levels. In contrast, CoCl2 at a concentration of 50 uM caused a significant decrease in ICAM-1 mRNA levels (40% of the control value; P < 0.004, n ; 4). These data indicate that in short-term exposure experiments the hard metal particles have no significant effect on the expression of selectin and ICAM-1 family of adhesion molecules. The pathophysiology of hard metal disease remains to be elucidated.
Heavy-metals; Metallic-dusts; Metal-dusts; Metal-compounds; Metallic-compounds; Tungsten-compounds; Cobalt-compounds; Genotoxic-effects; Cell-function; Cellular-function; Respirable-dust; Dust-particles; Particulate-dust
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Abstract; Conference/Symposia Proceedings
Molecular Biology of the Cell