Acute pulmonary responses to intratracheal instillation of asbestos: amelioration by tetrandrine.
Ma-JYC; Barger-MW; Ma-JKH; Castranova-V
FASEB J 1997 Feb; 11(3):A128
Animal exposure to various forms of asbestos, including crocidolite (amphibole) and chrysotile (serpentine) can produce pulmonary fibrosis. Tetrandrine (TT), a bisbenzylisoquinoline alkaloid, has shown the ability to inhibit silica-induced activation of alveolar macrophages (AM) as well as the fibrosis which results. In the present study, we investigated the effects of TT on crocidolite and chrysotile induced pulmonary responses in rats. Rats were given a single intratracheal instillation of 5mg/kg asbestos and sacrificed 7 days later. TT was administered orally (I8mg/kg) 3 times/week beginning 7 days prior exposure. In asbestos treated rats, pulmonary inflammation was demonstrated by an increased number of lymphocytes and neutrophils in the alveolar lavage fluid (LF). Cytotoxicity was evidenced by elevated lactate dehydrogenase (LDH) in the LF. In addition, enhanced amounts of IL-I and nitric oxide (NO) were released from AM harvested from exposed rats. TT treatment did not significantly affect the crocidolite-induced inflammation, cytotoxicity or IL-I and NO release. In contrast, TT treatment markedly ameliorated the chrysotile-mediated increase in lymphocytes, IL-I secretion, and NO production from AM by 33%. These results suggest that TT partially inhibits AM-orchestrated inflammatory processes which result from chrysotile exposure.
Asbestos-fibers; Asbestosis; Silica-dusts; Particulate-dust; Dust-particles; Dust-inhalation; Alveolar-cells; Laboratory-animals; Animal-studies; Cytotoxic-effects
12001-28-4; 12001-29-5; 10024-97-2; 1332-21-4
Abstract; Conference/Symposia Proceedings
The FASEB Journal