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Non-random chromosomal changes in high and low invasive tumor cells derived from early passage mouse lung adenocarcinoma cell cultures.

Ensell-MX; Reynolds-SH; Jefferson-AM; Lowry-DT; Kashon-ML; Tyson-FL; Senft-JR; Sargent-LM
Proceedings of the 93rd American Association for Cancer Research Annual Meeting, April 6-10, 2002, San Francisco, California. Philadelphia, PA: American Association for Cancer Research, 2002 Apr; 43:634
In an attempt to identify chromosomal changes that may be involved with invasive and/or metastatic properties, we used Spectral Karyotyping to analyze nine pairs of high and low invasive tumor cell cultures derived from early passage (3 to 5 passages from the primary tumor) mouse lung adenocarcinoma cell lines. The high and low invasive cultures were obtained by a Gel Matrix technique. The cells with high invasive property penetrate the gel matrix while the cells with low invasive property remain on the surface. Seven of the nine original lung adenocarcinomas were from the A/J mouse, a strain highly susceptible to lung carcinoma. Two of the nine original lung adenocarcinomas were from B6C3F1 mice, an F1 hybrid resulting from a cross between two resistant strains, C57BU6J and C3H/Hen. At least twenty metaphase spreads were observed for each culture: A chromosomal event that occurred in over 40% of the cells from a specific culture was scored as a positive event. Regression Tree analysis showed that duplication of chromosome 1 is significant in high invasive cultures (9/9) compared to low invasive cultures (3/9). Mapping with fluorescent labeled genomic probes identified the minimal region of duplication to be 73 to 94 centimorgan on chromosome 1, which includes a candidate Cyclooxyenase 2 (C0X2) gene. Further study for the expression of C0X2 is under investigation. Duplication of chromosome 15 was also found to be significantly higher in high invasive (8/9) than in low invasive (4/9) cultures. Our results indicate that chromosome 1 and 15 may be involved with the invasiveness and the candidate genes on these chromosome regions may play role for invasive and/or metastatic properties.
In-vitro-study; DNA-damage; Chromosome-damage; Chromosome-disorders; Cancer; Cancer-rates; Genetic-factors; Genetics; Laboratory-animals; Animals; Animal-studies; Models; Lung-cancer; Respiratory-system-disorders; Pulmonary-system-disorders
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Proceedings of the 93rd American Association for Cancer Research Annual Meeting, April 6-10, 2002, San Francisco, California
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