Oxidative stress, pulmonary toxicity and progressive fibrosis induced by exposure to single-walled carbon nanotubes.
Free Radicals in Montevideo, Montevideo, Uruguay, September 2-6, 2007. Montevideo, Uruguay: The Center for Free Radical and Biomedical Research - Uruguay, 2007 Sep; :62
Nanotechnology is a fast growing field with emerging development of modern materials for electronics and information technology industries, cosmetics, health and medicine and in a variety of manufactured goods. Single-Walled Carbon Nanotubes (SWCNT) with unique physico-chemical, electronic and mechanical properties are novel materials of technological importance. Pulmonary exposure to SWCNT revealed toxicity within the range of doses relevant to exposure limits established by regulatory agencies for fine size carbon particles. In C57BL/6 mice exposed to SWCNT, pulmonary toxicity was associated with a dose-dependent augmentation of biomarkers of cell injury and oxidative stress quantified by cell counts, total protein, lactate dehydrogenase and g-glutamyltranspeptidase activities, reduced level of GSH, total antioxidant reserve along with the accumulation of lipid peroxidation products found in bronchoalveolar lavage (BAL) fluid and in the lung. Markers of pulmonary cytotoxicity corresponded to early development of acute inflammation, collagen accumulation, and progressive fibrosis. In mice maintained on vitamin E deficient diet, SWCNT significantly enhanced collagen deposition as compared to the vitamin E sufficient group. Overall, our data suggest that pharyngeal aspiration of SWCNT elicited a robust acute inflammatory response with early onset of progressive pulmonary fibrosis whose expression and severity was associated with the intensity of oxidative stress in the lung of the exposed C57BL/6 mice.
Particle-aerodynamics; Particulate-dust; Particulates; Pulmonary-congestion; Pulmonary-disorders; Pulmonary-system; Pulmonary-system-disorders; Respiration; Respiratory-hypersensitivity; Respiratory-irritants; Respiratory-rate; Respiratory-system-disorders; Lung; Lung-cells; Lung-disorders; Lung-fibrosis; Lung-irritants; Laboratory-animals; Nanotechnology
Abstract; Conference/Symposia Proceedings
Free Radicals in Montevideo, September 2-6, 2007, Montevideo, Uruguay
University of Pittsburgh at Pittsburgh