NIOSHTIC-2 Publications Search

Creatinine measurements in 24 h urine by liquid chromatography-tandem mass spectrometry.

Park E-K; Watanabe T; Gee SJ; Schenker MB; Hammock BD

J Agric Food Chem 2008 Jan; 56(2):333-336

https://doi.org/10.1021/jf072433s

20033680

A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 microL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine- d3 were observed at m/z 114 > 44 and m/z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated (R2 = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods.

Agricultural-chemicals; Chemical-composition; Chemical-hypersensitivity; Chemical-reactions; Urinalysis; Exposure-levels; Urine-chemistry; Sample-preparation; Sampling-methods

Department of Entomology and UCD Cancer Research Center, University of California, Davis, California 95616

JAFCAU

67-56-1

20080123

Journal Article

Agriculture; Cooperative Agreement

2008

Cooperative-Agreement-Number-U50-OH-007550

2

0021-8561

Agriculture, Forestry and Fishing

Journal of Agricultural and Food Chemistry

CA

University of California - Davis