Use of a human microsomal library for metabolite elucidation, assay development and biological monitoring of occupationally relevant compounds.
Striley-CAF; Snawder-JE; Biagini-RE
Abstr Pap - Am Chem Soc 2001 Apr; 221(Part 2):U59
Immunochemical biomonitoring is an effective tool for assessing exposure to agrochemicals. Effective biomonitoring requires knowledge of agent-specific human pharmacokinetics We have procured a library of human tissue subfractions (hepatic, renal, pulmonary and jejunal) to serve as an invitro surrogate of human metabolism. Characterization of these tissues for Phase I and Phase II metabolic enzymes has been performed to elucidate variation in metabolism. The impact of individual variation on the metabolism of occupational/environmental chemicals can be estimated by accurately quantifying the expression of the pertinent enzyme(s) in human tissues. These data can be combined with data describing the specific activity of CYP forms towards substrates to develop metabolic rates, and their ranges, useful for the development of suitable biomarkers to estimate exposure to xenobiotics. Microsomal assay and HPLC analysis was used to determine human metabolism of azinphosmethyl, piperalin and bromopropane. Candidate biomarkers were then used to develop ELISAs for urinary analysis
Analytical-processes; Analytical-chemistry; Agricultural-chemicals; Biological-monitoring; Environmental-health-monitoring; Humans; Cell-metabolism; Biomarkers; Urinalysis; Urine-chemistry
Cynthia AF Striley, Biomonitoring and Health Assessment Branch, National Institute for Occupational Safely and Health, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
Abstract; Conference/Symposia Proceedings
Abstracts of Papers - American Chemical Society