Analysis of immunotoxicity by enumeration of antibody-producing B cells.
Anderson SE; Munson AE; Mead BJ
Current protocols in toxicology. Costa LG, Hodgson E, Reed DJ, Maines MD, Sassa S, Sipes IG, Bradfield C, Lawrence DA, Morgan KS, eds. New York: Wiley and Sons, Inc., 2004 Jan; 2(Suppl 20):18.11.1-18.11.19
The plaque-forming cell (PFC) assay measures the humoral immune response mediated by the concerted actions of antigen-presenting cells, T lymphocytes, and B lymphocytes. The most common form of the plaque method is used for the detection of murine primary IgM antibodies directed against the T cell-dependent sheep red blood cell (sRBC) surface antigens. Research has shown that the PFC response to sRBC is not only an excellent monitor of the primary effector function of the B cell, but that it may be the most sensitive immune parameter currently available to identify chemical perturbation. Several modifications and variations of the PFC assay are described in detail in this unit.
Diseases; Risk-analysis; Risk-factors; Immune-system; Infectious-diseases; Immunotoxins; Immunological-tests; Epidemiology; Quantitative-analysis; Models; Immunoglobulins; Qualitative-analysis; Immune-system-disorders; Immune-system; Cell-culture-techniques; Cell-function; Chemical-properties
Costa LG; Hodgson E; Reed DJ; Maines MD; Sassa S; Sipes IG; Bradfield C; Lawrence DA; Morgan KS
Current protocols in toxicology