Investigation of protein expression in magnetic field-treated human glioma cells.
Kanitz MH; Witzmann FA; Lotz WG; Conover D; Savage RE Jr.
Bioelectromagnetics 2007 Oct; 28(7):546-552
We previously reported phenotypic changes in human breast cancer cells following low-level magnetic field (MF) exposure. Here proteomic methods were used to investigate the biochemical effect of MF exposure in SF767 human glioma cells. Protein alterations were studied after exposure to 1.2 microTesla (µT) MF [12 milliGauss (mG), 60 Hertz (Hz)] +/- epidermal growth factor (EGF). SF767 cells were exposed for 3 h to sham conditions (<0.2 µT ambient field strength) or 1.2 µT MF (+/-EGF; 10 ng/ml). Solubilized protein fractions (sham; 1.2 µT; sham + EGF; 1.2 µT + EGF) were loaded for electrophoresis by 2D-PAGE and stained using a colloidal Coomassie blue technique to resolve and characterize the proteins. Protein patterns were compared across groups via Student's t-test using PDQUEST software. Cell profiles revealed significant alterations in the spot density of a subset of treated cells. Automated spot excision and processing was performed prior to peptide mass fingerprinting proteins of interest. Fifty-seven proteins from the detectable pool were identified and/or found to differ significantly across treatment groups. The mean abundance of 10 identified proteins was altered following 1.2 µT exposure. In the presence of EGF six proteins were altered after low magnetic field treatment by increasing (4) or decreasing (2) in abundance. The results suggest that the analysis of differentially expressed proteins in SF767 cells may be useful as biomarkers for biological changes caused by exposure to magnetic fields.
Genes; Proteins; Genetic-factors; Exposure-levels; Carcinogens; Carcinogenicity; Carcinogenesis; Magnetic-fields; Cell-function; Biomarkers;
Author Keywords: ELF magnetic field; glioma; proteomics; two-dimensional gel electrophoresis; protein expression
M.H. Kanitz, MGMT, BHAB, DART, NIOSH, 4676 Columbia Parkway, MS/C-23, Cincinnati, OH 45226-1998
Research Tools and Approaches: Exposure Assessment Methods