Methyl and phenyl esters and thioesters of carboxylic acids as surrogate substrates for microassay of proteinase K esterase activity.
Borhan-B; Hammock-B; Seifert-J; Wilson-BW
Anal Bioanal Chem 1996 Feb; 354(4):490-492
The development of a microassay for proteinase K esterase activity with carboxylic acid esters is reported using novel substrates of the general formula R-C(O)-XR. Highest rates of hydrolysis have been obtained with the O-phenyl esters CH3(CH2)n = 1 - 2-S-(CH2)n = 1 - 2 C(O)-O-phenyl and their thioester analogs in studies where R, X and R have been varied. The phenol release has been measured with 4-aminoantipyrine and potassium ferricyanide to determine the rates of O-phenyl ester hydrolyses. Thioester hydrolyses have been monitored continuously with 5,5-dithio-bis (2-nitrobenzoic acid).
Chemical-analysis; Chemical-composition; Chemical-indicators; Chemical-properties; Chromatographic-analysis; Spectrographic-analysis; Chemical-extraction; Chemical-reactions; Chemical-structure; Chemical-synthesis
Borhan, B: Department of Chemistry, University of California, 95616 Davis, CA, USA
Analytical and Bioanalytical Chemistry
University of California - Davis