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Identification of human urinary metabolites of acetochlor in exposed herbicide applicators by high-performance liquid chromatography-tandem mass spectrometry.
Barr-DB; Hines-CJ; Olsson-AO; Deddens-JA; Bravo-R; Striley-CAF; Norrgran-J; Needham-LL
J Expo Sci Environ Epidemiol 2007 Sep; 17(6):559-566
Acetochlor is a preemergent chloroacetanilide herbicide used to control annual grasses and small-seeded broadleaf weeds. It is the second most abundantly applied herbicide on corn crops in the United States; however, human metabolites associated with known exposure to acetochlor have not been positively identified and confirmed. We positively identified acetochlor mercapturate (ACM) as a metabolite of acetochlor in urine samples collected during a 24-h period from custom ( commercial) applicators who had applied acetochlor on either the day of or the day before urine collection. Concentrations in applicator urine samples ranged from 0.5 to 449 mu g/l (0.3-121 mu g/g creatinine). We found that ACM accounted for as much as 42% of the total acetochlor-derived metabolites; however, as the exposure level decreased ( based on total acetochlor metabolite level), ACM became a less abundant metabolite of acetochlor (< 17%). Unmetabolized acetochlor was also measured in the urine samples analyzed. At high exposures ( classified as 4100 mu g/l), acetochlor accounted for about 0.8% of the total excreted acetochlor metabolites( similar to 2% of the ACM concentrations). At lower exposures ( classified as ACM < 10 mu g/l), ACM and acetochlor concentrations were similar. Additionally, we tentatively identified another acetochlor metabolite that appeared to be important at low levels of exposure.
Biological-factors; Biological-monitoring; Agricultural-industry; Agriculture; Herbicides; Urine-chemistry; Metabolites; Work-areas; Work-environment; Pesticides-and-agricultural-chemicals; Pesticides; Biomarkers
Dr. Dana B. Barr, Centers for Disease Control and Prevention, National Center for Environmental Health, 4779 Buford Highway, Mailstop F17, Atlanta, GA 30341, USA
Issue of Publication
Agriculture, Forestry and Fishing
Journal of Exposure Science and Environmental Epidemiology
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division