Exposure to NRL allergens plays a key role in inducing NRL allergy. We therefore compared the relative abilities of air and dust sampling to detect NRL allergen contamination in a medical facility. High volume area air samples and vacuumed bulk surface dust samples were collected onto Teflon filters in several hospital and clinic settings. Control samples were collected in a vacant office where NRL was not used. NRL allergen levels in saline filter extracts were determined by inhibition of an immunoassay for NRL-specific human IgE (Pharmacia-Upjohn Diagnostics, Uppsala, Sweden). Threshold limit of detection (LCD) for the air and dust samples was determined as the mean level in the vacant office plus 3 standard deviations. Thus, NRL allergen contamination in a medical facility was more often detected by sampling of bulk surface dust than by area air sampling.
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