Bovine red blood cell ghost cholinesterase as a monitoring standard.
Arrieta-D; Ramirez-A; DePeters-E; Bosworth-D; Wilson-BW
Bull Environ Contam Toxicol 2003 Sep; 71(3):447-452
We conducted a comparison test at the request of the EP A with several clinical laboratories on blood ChE of rats exposed to various levels of an organophosphate pesticide (Wilson et al, 1996); we found that the conduct of the assay introduced inaccuracies into the clinical laboratory procedures. For example, not correcting for a transient thiol oxidase activity present in rodent red blood cells (RBC) introduced an indeterminate error in the results. Subsequently, while working with California state agencies and clinical laboratories licensed to monitor farm worker blood ChEs, we found conditions of a commonly used version of the Ellman assay (formerly Boehringer-Mannheim kit, Catalog No. 450035, Boehringer Mannheim Corporation, Indianapolis, IN) decreased the activity of the assay by approximately 40 percent (Wilson et a!. 1997). In a comparison project with several clinical laboratories, only two of nine that participated achieved satisfactory results assaying ChE in human blood (Wilson et al. 2002). These findings led to a change in the California state regulations requiring ChE assays be convertible to values obtained from an optimized Ellman assay (Wilson et al. 2002). As part of a project to help standardize and optimize ChE assays for clinical laboratories, we developed a RBC ghost AChE standard using cows. This brief report describes the RBC ghosts and their enzymatic properties.
Cholinesterase-inhibitors; Monitoring-systems; Blood-analysis; Agricultural-workers; Agriculture; Pesticides; Hazards; Biomarkers; Bioassays; Laboratory-animals; Enzyme-activity; Enzymatic-effects
D. Arrieta, Department of Environmental Toxicology, University of California, Davis, CA 95616, USA
Bulletin of Environmental Contamination and Toxicology
University of California - Davis