NIOSHTIC-2 Publications Search

Factors in standardizing automated cholinesterase assays.

Wilson BW; Padilla S; Henderson JD; Brimijoin S; Dass PD; Elliot G; Jaeger B; Lanz D; Pearson R; Spies R

J Toxicol Environ Health, A 1996 Jun; 48(2):187-195

https://doi.org/10.1080/009841096161429

20032546

A scientific panel assembled by the U.S. Environmental Protection Agency (EPA) determined that variability in cholinesterase (ChE) activities in the agency's pesticide/animal study database likely was due to a lack of accepted guidelines for ChE methodology. A series of trials was held in which participating laboratories measured ChE activity in blood and brain samples from untreated and pesticide-treated rats using a colorimetric assay method. The degree of inhibition of ChE activity in plasma and brain samples compared to controls was consistent among most of the laboratories. The ChE activity in erythrocyte samples differed more between laboratories due to a high blank, low erythrocyte AChE activity and hemoglobin absorption at the wavelength of the assay. Strategies are suggested for minimizing the variability of ChE activity in hemoglobin-rich samples.

Chemical-analysis; Pesticides; Pesticides-and-agricultural-chemicals; Colorimetry; Erythrocytes; Sampling; Sampling-methods; Blood-analysis; Analytical-processes; Analytical-methods; Analytical-instruments

Barry W. Wilson, Departments of Environmental Toxicology and Avian Sciences, University of California, Davis, California, USA

JTEHD6

19960607

Journal Article

1055222

Cooperative Agreement

1996

Cooperative-Agreement-Number-U07-CCU-906162

2

1528-7394

Journal of Toxicology and Environmental Health, Part A: Current Issues

CA; MN; DE; KS; MD; NJ; VA; WA

University of California - Davis