Antioxidant activity of Vaccinium stamineum: exhibition of anticancer capability in human lung and leukemia cells.
Wang-SY; Feng-RT; Bowman-L; Lu-YJ; Ballington-JR; Ding-M
Planta Med 2007 May; 73(5):451-460
Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity and anticancer properties in JB6 P+ mouse epidermal cells, human lung and leukemia cells. Deerberries contain potent free radical scavenging activities. Pretreatment of JB6 P+ mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappa B) induced by either 12-0-tetradecanoylphorbol 13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK 1/2, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL60 cancer cells and human lung epithelial cancer A549 cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-kappa B activity, inhibition of HL-60 cells and cancer A549 cells proliferation and induction of apoptotic in human leukemia HL-60 cancer cel Is may be mediated through the ERKs and MEK 1/2 signal pathway.
Analytical-processes; Analytical-methods; Sensitivity-testing; Animal-studies; Lung-cancer; Lung-cells; Lung-disease; Lung-tissue; Cancer; Humans; Leukocytes; Antigens; Antibody-response
SY Wang, USDA ARS, Beltsville Agriculture Research Center, Agriculture Research Service, Fruit Laboratory, Beltsville, MD 20705