Cell growth arrest is an important mechanism in maintaining genomic stability and integrity in response to environmental stress. Using human lung epithelial cell line, A649, the present study investigates the role of reactive oxygen species (R08) and ERK and p38 protein kinase in vanadate-induced cell growth arrest. Exposure of the cells to vanadate led to cell growth arrest at G2/M phase and caused up-regulation of p21 and phpho-cdc2 and degradation of cdc25C. Vanadate activated phosphorylation of ERK and p38. PD98059 and SB202190 not only decreased phosphorylation of ERK and p38 activated by vanadate, also inhibited vanadate-induced G2/M arrest, up-regulation of p21 an4 cdc2 and degradation of cdc25C. Cellular reduction of vanadate generated hydroxyl radical as determined by electron spin resonance (ESR) and superoxide radical and hydrogen peroxide as determined by flow cytometry and confocal microscopy in combination with specific antioxidant enzymes, Buperoxide dismutase and catalase. Among ROS generated by cellular reduction of vanadate, both hydroxyl radical and hydrogen peroxide play an important role in vanadate-induced activation of ERK and p38, up-regulation of p21 and phospho-cdc2, and degradation of cdc25C. These results suggest that cellular reduction of vandate generates hydroxyl radical and hydrogen peroxide via superaxide anion as an intermediate. ROS activate ERK and p38, which in turn up-regulate, p21 and cdc2 and cause degradation of cdc25C leading to cell growth arrest at G2/M phase. ROS affect different MAPK family members and cell growth regulatory proteins with different potencies.
Links with this icon indicate that you are leaving the CDC website.
The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website.
Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website.
You will be subject to the destination website's privacy policy when you follow the link.
CDC is not responsible for Section 508 compliance (accessibility) on other federal or private website.
For more information on CDC's web notification policies, see Website Disclaimers.
CDC.gov Privacy Settings
We take your privacy seriously. You can review and change the way we collect information below.
These cookies allow us to count visits and traffic sources so we can measure and improve the performance of our site. They help us to know which pages are the most and least popular and see how visitors move around the site. All information these cookies collect is aggregated and therefore anonymous. If you do not allow these cookies we will not know when you have visited our site, and will not be able to monitor its performance.
Cookies used to make website functionality more relevant to you. These cookies perform functions like remembering presentation options or choices and, in some cases, delivery of web content that based on self-identified area of interests.
Cookies used to track the effectiveness of CDC public health campaigns through clickthrough data.
Cookies used to enable you to share pages and content that you find interesting on CDC.gov through third party social networking and other websites. These cookies may also be used for advertising purposes by these third parties.
Thank you for taking the time to confirm your preferences. If you need to go back and make any changes, you can always do so by going to our Privacy Policy page.