Molecular and morphologic determinanats of organic dust exposures in alveolar macrophage (AM) cells.
Cosma-GN; Davis-WF; Ufferfilge-JE; Olenchok-SA; Beard-ML
Toxicologist 1995 Mar; 15(1):96
To understand the pathology of pulmonary diseases after exposures to airborne dusts, we have previously measured acute-phase gene induction in rat/mouse lung after inhalation of respirable particulates. We have now measured in vitro responses of target AM cells to grain dust (GD) extracts and specific etiologic agents (bacterial endotoxin and fungal mycotoxins) found in elevator wheat samples. Area samples of respirable wheat dust were collected onto filters by personal pumps during routine elevator operations. Endotoxin levels were, quantitated by chromogenic LAL assay, and fungi identified by standard mycologic culture. Rat AM cell line NR8383 displayed greatly reduced cell growth and elevated toxicity in response to purified endotoxin vs. Alternaria mycotoxins. When cells were exposed to G D extracts, dose-responses most closely resembled those of endotoxin alone, suggesting a pivotal role for this febrile agent in the pathophysiology of dust-related pulmonary diseases, induction of the metallothionein gene was also detected by Northern blot analyses. Further acute-phase gene induction and oxidative burst analyses are in progress.
Laboratory-animals; Animal-studies; Dust-particles; Dust-exposure; Particulate-dust; Airborne-particles; Pulmonary-system-disorders; Respiratory-system-disorders; Respirable-dust; Lung-irritants; Lung-disorders; Organic-dusts; Bacterial-dusts; Fungi
The Toxicologist. Society of Toxicology 34th Annual Meeting, March 5-9,1995, Baltimore, Maryland