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Salivary concentrations of atrazine reflect free atrazine plasma levels in rats.

Lu C; Anderson LC; Morgan MS; Fenske RA

J Toxicol Environ Health, A 1998 Feb; 53(4):283-292

https://doi.org/10.1080/009841098159286

20032234

The protein binding of atrazine in plasma and its effect on salivary excretion of atrazine was determined in male Sprague-Dawley rats. The degree of protein binding of atrazine was determined at 3 steady-state plasma concentrations, 50, 150, and 250 microg/L, using an ultrafiltration technique. In total, 48 arterial blood samples were collected from 18 rats; 38 of 48 blood samples had their time-matched whole saliva samples. The average protein binding of atrazine ranged from 18% to 37%; however, it was not significantly different across the 3 steady-state plasma concentrations nor among the individual rats. Overall, 26% of atrazine was bound to plasma proteins and not available for transport from blood into saliva. Protein binding of atrazine in plasma was not correlated with total atrazine plasma concentration nor with free atrazine plasma concentration, which indicates that the protein-bound fraction of atrazine is independent of plasma concentration within the range measured in this study (30-400 microg/L). The average saliva/plasma (S/P) concentration ratio of atrazine increased from 0.7 using total atrazine plasma concentration to 0.94 (S/fP) when free atrazine plasma concentrations calculated as 26% of protein binding was used. Salivary concentration was highly correlated with free atrazine plasma concentration. The results suggest that salivary concentration of atrazine not only reflects its total plasma level but accurately measures the portion of atrazine (free atrazine) in plasma, which may be of toxicological significance

Chemical-analysis; Chemical-composition; Protein-synthesis; Proteins; Salivary-glands; Sampling; Blood-plasma; Blood-samples; Blood-sampling; Toxic-dose

Department of Environmental Health, School of Public Health and Community Medicine, University of Washington, Seattle 98195-7234

JTEHD6

1912-24-9

19980220

Journal Article

1358789

Grant

1998

Grant-Number-T42-CCT-010418

4

1528-7394

Journal of Toxicology and Environmental Health, Part A: Current Issues

WA; CA

University of Washington