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Grid-immunoplotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity.
Reese-G; Schmechel-D; Ayuso-R; Lehrer-SB
J Chromatogr B 2001 May; 756(1-2):151-156
Grid-immunoblotting is a procedure that allows the simultaneous testing of up to 20 different antibodies such as monoclonal antibody-containing hybridoma supernatants or human sera for specific antibodies to up to 20 different antigens or allergens on a single sheet of nitrocellulose membrane. Since only 150 to 200 mul of antibody-containing solution are required this technique is uniquely suited to test growing hybridomas and small amounts of sera (e.g. mouse and children's sera). Compared to a standard ELISA, approximately ten times less antibody is needed to obtain the same information.
Immunological-tests; Immunology; Allergies; Allergic-reactions; Biological-agents; Blood-analysis; Blood-sampling; Blood-serum; Blood-tests
Issue of Publication
Journal of Chromatography B: Biomedical Applications
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division