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Urinary benzo[a]pyrene and its metabolites as molecular biomarkers of asphalt fume exposure characterized by microflow LC coupled to hybrid quadrupole time-of-flight mass spectrometry.
Wang-JJ; Frazer-DG; Stone-S; Goldsmith-T; Law-B; Moseley-A; Simpson-J; Afshari-A; Lewis-DM
Anal Chem 2003 Nov; 75(21):5953-5960
As a step to study the health effects of asphalt fume exposure, an analytical method was developed to characterize benzo[a]pyrene and its hydroxy metabolites in the urine of asphalt fume-exposed rats. This method is based on microflow liquid chromatography (LC) coupled to hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometry (Q-TOFMS). Twenty-four female Sprague-Dawley rats were used in the experiment, with 8 as controls and 16 exposed to asphalt fumes in a whole-body inhalation chamber for 10 days (4 h/day). Generated at 150 C, the asphalt fume concentration in the animal exposure chamber ranged 76-117 mg/m3. In the urine of the asphalt fume-exposed rats, benzo[a]pyrene and its metabolites of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-7,8-dihydrodiol(+/-), and benzo[a]pyrene-7,8,9,10-tetrahydrotetrol(+/-) were identified, and their concentrations were determined at 2.19 +/- 0.49, 16.17 +/- 0.3, 6.28 +/- 0.36, and 29.35 +/- 0.26 ng/100 mL, respectively. The metabolite concentrations from the controlled group, however, were either under the detection limits or at a relatively very low level (0.19 +/- 0.41 ng/100 mL for benzo[a]pyrene-7,8,9,10-tetrahydrotetrol metabolite). The results clearly indicate that the benzo[a]pyrene and its hydroxy metabolites were significantly elevated (p < 0.001) in the urine of asphalt fume-exposed rats relative to controls. The study also demonstrated that the combination of microflow LC separation and collision-induced dissociation leading to a characteristic fragmentation pattern by hybrid Q-TOFMS offers a distinct advantage for the identifications and characterizations of the benzo[a]pyrene metabolites.
Analytical-methods; Asphalt-fumes; Benzopyrenes; Chromatographic-analysis; Mass-spectrometry; Laboratory-animals; Animal-studies; Animals; Exposure-assessment; Metabolites; Hydroxy-compounds
Jin J. Wang, National Institute for Occupational Safety and Health, Health Effects laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505
Issue of Publication
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division