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Restraint-induced modification of Langerhans cell morphology and migration in BALB/c mice is dependent upon timing of restraint.
Flint-M; Abrigo-B; Tinkle-S
J Invest Dermatol 2001 Aug; 117(2):446
The timing of a stressful event with respect to antigen exposure affects the development of the immune response. We have reported that restraint applied to a naive mouse prior to sensitization (day 1) decreased chemical-induced changes in ear swelling and lymphocytosis and restraint applied during sensitization (days 1 and 2) or prior to challenge (day 6), increased ear swelling. We hypothesized that these dichotomous effects of restraint would be reflected in changes in Langerhans cell (LC) migration and morphology. To compare the effects of restraint on LC in naive and DNFB-treated mice, we sensitized mice onto the back with 0.5% DNFB on days 1 and 2 and challenged onto the ears with 0.25% DNFB on day 6. Mice were restrained for 2 h immediately prior to chemical application on day 1 or on day 6 prior to challenge. LC morphology and migration were examined in epidermal sheets by counting the number of FITC-conjugated Ia stained cells per mm2 at 24 h after DNFB-challenge. To assess the effect of restraint on T cell proliferation in vivo, 24 h following challenge, T lymphocyte proliferation was examined using the local lymph node assay. We determined that DNFB induced a significant reduction in the number of epidermal LC at 24 h. Restraint on day 1 resulted in the retention of LC in the epidermis and the LC had longer dendritic processes than nonrestrained mice. However, LC from mice restrained on day 6 demonstrated a dendritic cell like-morphology similar to nonrestrained mice but showed a significant reduction in epidermal LC numbers 24 h after DNFB challenge. Furthermore, we demonstrated a significant two-fold decrease in T cell proliferation in mice restrained on day 1 and an elevation of T cell proliferation in mice restrained on day 6. These data suggest that acute restraint stress applied prior to sensitization modulates epidermal LC morphology and migration and T cell proliferation differently than restraint applied prior to challenge.
Cell-migration; Cell-morphology; Antigens; Immune-reaction; Immune-system; Animal-studies; Stress; Endocrine-system-disorders; Endocrine-function
Abstract; Conference/Symposia Proceedings
Issue of Publication
Journal of Investigative Dermatology
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division