Inhibition of glutathione synthesis does not increase oxidative stress in response to repetitive stretch-shortening contractions.
Hollander-MS; Baker-BA; Kashon-ML; Cutlip-RG
FASEB J 2007 Apr; 21(6)(Suppl):A1309
We investigated effects of glutathione (GSH) synthesis inhibition on the oxidative stress status of tibialis anterior (TA) muscles in young (12 wk) and old (30 mo) Fisher 344 x Brown Norway F1 male rats. L-Buthionine (S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis, was administered to rats in the drinking water at 10mM. Left dorsiflexor muscles of old and young rats were exposed 3 times a week for 4.5 weeks to a protocol of 80 maximal stretch-shortening contractions (SSC) per exposure in vivo. TA muscle response was characterized by measurement of malondialdehyde (MDA) as a marker of lipid peroxidation, which is used as an indicator of oxidative stress. GSH was measured to indicate effects of treatment. GSH levels were lower in BSO treated rats compared to unsupplemented rats in old and young (p < 0.05) indicating that BSO was effective in decreasing GSH. The LTA presented with a higher MDA level in old BSO treated rats compared to the RTA (p < 0.05). LTAs from old rats had higher MDA compared to young rats in the BSO, unsupplemented, and anesthesia groups (all p < 0.05). Based on our findings, it does not appear as if decreased GSH levels or prolonged anesthesia affects the oxidative stress status of the TA muscle after exposure to repetitive SSCs.
Cellular-function; Cellular-structures; Cell-differentiation; Cell-function; Cell-biology; Animal-studies; Muscle-cells; Muscle-contraction; Muscle-physiology; Muscle-stress; Muscle-tissue; Musculoskeletal-system; In-vivo-studies
Health Effects Laboratory Division, CDC National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV, 26505
Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
The FASEB Journal