Eukaryotic translation elongation factor 1delta overexpressing cells are resistant to cadmium-induced toxicity and apoptosis.
Othumpangat-S; Umbright-C; Joseph-P
Toxicologist 2007 Mar; 96(1):396
The role of Eukaryotic translation elongation factor 1sigma (eEF1sigma) in cadmium-induced cytotoxicity and apoptosis was investigated. Transgenic cell line overexpressing the eEF1sigma gene were generated by transfecting HeLa cells with the expression vector pcDNA 3.1 containing human eEF1sigma cDNA. Stable transfectant cell line was developed using blasticidin (5mg/mL medium). Expression of eEF1sigma in the transgenic cell line was determined by real-time PCR and Western blot analysis. Cell line exhibiting 2 fold (eEF1sigma4) and 3 fold (eEF1sigma11) increases in eEF1sigma expression were developed. The vector alone transfected cell line served as the control. The transgenic cell line exhibited enhanced cell proliferation, overexpression of eEF1sigma, eIF4E and eEF2 genes compared with the control cell line. Cadmium-induced cytotoxicity was significantly lower in the eEF1sigma overexpressing cell line compared with the control. Cadmium induced apoptosis was also studied using the control and eEF1sigma overexpressing cell lines. The eEF1sigma overexpressing cell line exhibited significantly higher resistance to cadmium-induced apoptosis compared with the control cell line. Real-time PCR analysis of eEF1sigma overexpressing cell line exposed to cadmium showed significant induction of anti-apoptotic genes like Bad and Bcl-2, with a concurrent decrease in the expression of the pro-apoptotic gene, p53 compared with the control cell line. In summary, the eEF1sigma gene overexpressing cell line exhibited significant resistance to cadmium-induced cytotoxicity and apoptosis.
Risk-analysis; Risk-factors; Mathematical-models; Statistical-analysis; Toxic-effects; Cell-alteration; Cell-function; Cell-metabolism; Cell-morphology; Cell-transformation; Cellular-structures; Gene-mutation; Genes; Genetic-factors; Genetics; Genotoxic-effects; Genotoxicity; Cytotoxic-effects; Cytotoxins; Cytotoxicity
The Toxicologist. Society of Toxicology 46th Annual Meeting and ToxExpo, March 25-29, 2007, Charlotte, North Carolina