The utility of four types of Ciphergen Protein Chips were evaluated by comparing protein profiles in control, and in in vitro acrylamide or glycidamide exposed urine using Surface Enhanced Laser Desorption Ionization Time of Flight (SELDI-TOF) mass spectrometry. Acrylamide (CAS 79-06-1), a widely used industrial chemical, which also may be formed in thermally processed food, can produce peripheral neurotoxicity and reproductive effects. Acrylamide has been classified as a probable human carcinogen. Changes in the levels of potential protein biomarkers in serum and urine have been reported in exposure to toxic agents, and bioactivation of acrylamide to glycidamide (5694-00-8), a reactive epoxide, suggests that urinary protein levels would be affected in acrylamide exposure. This work was designed to identify molecular biomarkers in human urine for use to assess acrylamide exposure or detect early disease onset. Urinary proteins were processed on NP20, CM10, H50, and IMAC30 chips having normal phase, cationic exchange, hydrophobic, and ionic surface chemistries, respectively. The number of peaks and mean peak intensity varied significantly with IMAC30 > CM10 > NP20 > H50. Various factors were optimized during the development of binding protocols for each chip, including surface pretreatment, sample binding time and washing methods. The data from this work suggest that the detection and further characterization of urinary protein levels using SELDI-TOF mass spectrometry can provide specific proteomic- based biomarkers of exposure for acrylamide. Disclaimers: Mention of company names and/or products does not constitute endorsement by the Centers for Disease Control and Prevention (CDC). The findings and conclusions in this abstract have not been formally disseminated by the National Institute for Occupational Safety and Health and should not be construed to represent any agency determination or policy.
The Toxicologist. Society of Toxicology 46th Annual Meeting and ToxExpo, March 25-29, 2007, Charlotte, North Carolina