Multiplexed analyses of serum antibodies to Bacillus anthracis toxins from cases with cutaneous anthrax in the former Soviet Republic of Kazakhstan.
Sammons-DL; Smith-JP; Biagini-RE; Snawder-JE; Striley-CAF; Robertson-SK; MacKenzie-BA; Wilkins-PP; Quinn-CP
Abstr Pap - Am Chem Soc 2003 Sep; 226(Part 1):U90 044-AGRO
Bacillus anthracis (Ba) exotoxins, lethal factor (IF) and protective antigen (PA) are immunogenic, and exposure may be evaluated by measurement of specific antibodies by multiplexed fluorescence covalent microbead Immunoassay (FCMIA). Using sera from individuals with "confirmed" cutaneous cases of (N=7) of anthrax from the former Soviet Republic of Kazakhstan (Kaz) and 8 donor controls we found 47.9 +/- 37.4 and 19.0 +/- 16.3, 1.00 +/- 0.98 and 0.14 +/- 0.12 microg/ml anti-LF lgG and anli-PA IgG, respectively (Kaz and control; mean +/- Standard Deviation (SD]; both P<0.001). For anti-LF 19M and anti-PA 19M, we found 1107 +/- 999 and 265 +/- 174, 33.5 +/- 29.7 and 9.6 +/- 6.0 (Kaz and control; standard sera equivalent dilution +/- SD; P<0.001), respectively, These data Indicate that cutaneous anthrax Infection can induce significant levels of anti-LF and anti-PA IgG and IgM which can be measured by multiplexed FCMIA.
Exposure-levels; Exposure-assessment; Blood-analysis; Antibody-response; Dermatitis; Skin-exposure; Skin-absorption; Serological-techniques; Bacteria; Bacterial-disease
Deborah L. Sammons, Biomonitorlng and Health Assessment Branch, CDC/NIOSH, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226,
Abstract; Conference/Symposia Proceedings
Abstracts of Papers - American Chemical Society