IgE reactivity to Paecilomyces variotii antigens in fungal sensitized patients.
Green-BJ; Blachere-FM; Beezhold-DH; Weissman-DN; Hogan-MB; Wilson-NW; Schmechel-D
J Allergy Clin Immunol 2007 Jan; 119(1)(Suppl 1):S187
Rationale: Exposure to Paecilomyces variotii can be extremely high in occupational environments and has been shown to be a risk factor for a number of allergic and invasive diseases. The purpose of this study was to determine the prevalence of sensitization to P. variotii in an atopic population from West Virginia. Methods: We evaluated 102 atopic patients for immunoglobulin E (lgE)-mediated hypersensitivity to fungi. In addition to our routine panel of skin prick test (SPT) reagents, patients were also tested to several indoor fungi including P. variotii. All patients had symptoms consistent with allergic rhinitis or asthma. The presence of specific IgE against P. variotii was additionally determined using immunoblotting. Results: Of the 102 eligible patients, 68% had at least one positive SPT to various aeroallergen sources. Overall, 21/102 (21%) patients were SPT positive to at least one fungal extract. Of the patients with a positive SPTt6 fungi, 7/21 (33%) demonstrated sensitivity to P. variotii. One and two dimensional immunoblotting of P. variotii mycelial extracts revealed specific IgE against multiple antigens that ranged from 30 to 200 kDa. IgE reactivity was greatest towards 22 kDa, 33 kDa, 40 kDa, 46 kDa and 70 kDa antigens. Non-atopic serum was negative towards the P. variotii extract. Conclusions: The prevalence of fungal sensitization to P. variotii was common in this cohort of fungal sensitized patients. Cross-reactivity among fungi may partially explain our results; however, the results indicate P. variotii may be an important species to monitor for both occupational and non-occupational fungal exposures.
Fungi; Microorganisms; Allergens; Allergic-reactions; Allergies; Respiratory-irritants
Abstract; Conference/Symposia Proceedings
Journal of Allergy and Clinical Immunology