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Retinoid signaling in immortalized and carcinoma-derived human uroepithelial cells.
Waliszewski P; Waliszewska M; Gordon N; Hurst RE; Benbrook DM; Dhar A; Hemstreet GP 3rd
Mol Cell Endocrinol 1999 Feb; 148(1-2):55-65
This paper investigates the presence and functionality of retinoid signaling pathways in human urinary bladder carcinoma and SV40-immortalized uroepithelial cell lines. Only two of eight cell lines were proliferation-inhibited by 10 microM of either all-trans or 13-cis-retinoic acid. Transactivation of the CAT gene under control of a retinoid-responsive element demonstrated functionality of the signaling pathway in both sensitive cell lines and four of six resistant cell lines. Relative RT-PCR analysis of a panel of retinoid-responsive and inducible genes demonstrated changes in expression levels of all the genes in response to-retinoic acid treatment together with numerous aberrations dysregulations. We conclude that retinoid signaling may be a target for inactivation during tumorigenesis by uncoupling gene expression, proliferation and differentiation. Therefore retinoids are more likely to be effective for chemoprevention than for treatment of bladder carcinomas.
Risk-factors; Risk-analysis; Cancer; Chemotherapy; Tumorigens; Tumorigenesis; Bladder-cancer; Biomarkers; Carcinogens; Carcinogenesis; Carcinogenicity; Exposure-assessment; Bladder-disorders; Urogenital-system-disorders
Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA
Issue of Publication
Molecular and Cellular Endocrinology
University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
Page last reviewed: December 30, 2021
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