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Tape-strip sampling for measuring dermal exposure to 1,6-hexamethylene diisocyanate.

Authors
Fent KW; Jayaraj K; Gold A; Ball LM; Nylander-French LA
Source
Scand J Work Environ Health 2006 Jun; 32(3):225-240
NIOSHTIC No.
20031151
Abstract
This study describes the development and evaluation of a method for sampling layers of the stratum corneum for the quantitation of dermal exposure to 1,6-hexamethylene diisocyanate (HDI). HDI deposited on skin was collected by the removal of stratum corneum with adhesive tape, derivatized with 1-(2-methoxyphenyl)piperazine, and quantitated as the urea derivative (HDIU) by liquid chromatography-mass spectrometry (LC-MS). This LC-MS method was tested by analyzing tape spiked with HDI-containing products, then applied to tape samples collected from the skin of an auto-body shop worker exposed to polyurethane paint aerosols. The limits of detection and quantitation were 20 and 50 fmol per injection, respectively. The recovery of HDI from the tape was 99.3% [95% confidence interval (95% CI) 97.1-102]. HDIU was stable at -40 degrees C, degrading by 0.28% (95% CI 0.10-0.46) per day. Quantifiable amounts of HDI were observed in 42.6% of the first three successive tape-strip samples collected from 36 different sites on the skin of the worker. The amount of HDI recovered from the collection sites on skin, measured by summing the levels collected with three successive tape-strips, ranged from nondetectable to 1874 pmol. This study demonstrates that HDI on skin can be collected with tape-strips and quantified at occupational levels using LC-MS.
Keywords
Sampling; Sampling-methods; Occupational-exposure; Exposure-assessment; Skin-exposure; Mass-spectrometry; Workers; Worker-health; Aerosols; Aerosol-particles; Automotive-industry; Automobile-repair-shops; Author Keywords: exposure assessment; sampling method; skin exposure; tape-stripping
Contact
Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina at Chapel Hill, Rosenau Hall CB #7431, Chapel Hill, NC 27599-7431, USA
CODEN
SWEHDO
CAS No.
822-06-0
Publication Date
20060601
Document Type
Journal Article
Funding Type
Grant
Fiscal Year
2006
Identifying No.
Grant-Number-R01-OH-007598; Grant-Number-T42-OH-008673
Issue of Publication
3
ISSN
0355-3140
Source Name
Scandinavian Journal of Work, Environment & Health
State
NC
Performing Organization
University of North Carolina, Chapel Hill
Page last reviewed: May 5, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division