A two-stage cyclone personal aerosol sampler using microcentrifuge tubes for bioaerosol collection.
Lindsley-WG; Schmechel-D; Chen-BT
Proceedings of the Seventh International Aerosol Conference, September 10-15, 2006, St. Paul, Minnesota, USA.. Biswas P, Chen DR, Hering S, eds. Mount Laurel, NJ: American Association for Aerosol Research, 2006 Sep; :801-802
The two-stage sampler that was developed for this project successfully divides aerosol samples into appropriate size fractions for bioaerosol collection. At 3.5 lpm, the first stage will collect most large fungal spores, bacteria and agglomerates, while the second stage will collect most small fungal spores and bacteria. Very small material such as fungal fragments and viruses will be collected by the back-up filter, which also serves to protect the sampling pump from contamination. At 2 lpm, the sampler would still collect many bacteria and fungal spores, and the higher cut-off diameters may be useful for separating some species or separating individual spores from agglomerates. Current work aims to improve the sharpness of the second stage collection efficiency and the development of immunological and molecular sample processing techniques using experimental fungal aerosols. The general design and techniques given here could also be adapted to design aerosol samplers using larger disposable tubes for longer-term atmospheric and indoor air quality sampling.
Aerosols; Fungi; Sampling; Sampling-equipment; Sampling-methods
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
Biswas-P; Chen-DR; Hering-S
Disease and Injury: Infectious Diseases
Proceedings of the Seventh International Aerosol Conference, September 10-15, 2006, St. Paul, Minnesota, USA.