Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Standard practice for sampling and counting airborne fibers, including asbestos fibers, in the workplace, by phase contrast microscopy (with and option of transmission electron microscopy).

Rook-HL; Rothert-J; Nagda-NL; Magee-RJ; Webber-JS; Danchik-RS; Ashley-K; Lewis-RG; Puzak-JC
Annual Book of ASTM Standards. West Conshohocken, PA: ASTM International, ASTM D 7201-06, 2006 Jul; 11.07:1-24
1.1 This practice describes the determination of the concentration of fibers, expressed as the number of such fibers per millilitre of air, using phase contrast microscopy and optionally transmission electron microscopy to evaluate particulate material collected on a membrane filter in the breathing zone of an individual or by area sampling in a specific location. This practice is based on the core procedures provided in the International Organization for Standardization (ISO) Standard ISO 8672 (1), the National Institute for Occupational and Health (NIOSH) Manual of Analytical Methods, NIOSH 7400 (2), and the Occupational Safety and Health Administration (OSHA) Method ID 160 (3). This practice indicates the important points where these methods differ, and provides information regarding the differences, which will allow the user to select the most appropriate procedure for a particular application. However, selecting portions of procedures from different published methods generally requires a user to report that they have used a modification to a method rather than claim they have used the method as written. 1.2 The practice is used for routine determination of an index of occupational exposure to airborne fibers in workplaces. Workplaces are considered those places where workers are exposed to airborne fibers including asbestos. Additional information on sampling strategies, sample collection (including calibration) and use of sample results for asbestos abatement projects is provided in a standard Practice for Air Monitoring for Management of Asbestos-Containing Materials (WK 8951) currently being considered by ASTM subcommittee E06.24. A further practice has been approved for the specific purpose of sampling and counting airborne fibers in mines and quarries (Practice D 7200), although the practice herein may also be used for this purpose. The current practice may be used as a means of monitoring occupational exposure to asbestos fibers when asbestos fibers are known a priori to be present in the airborne dust. The practice gives an index of airborne fiber concentration. This practice may be used in conjunction with electron microscopy (See Appendix X1) for assistance in identification of fibers. This practice may be used for other materials such as fibrous glass, or man-made mineral fibers by using alternate counting rules (see Annex A4). 1.3 This practice specifies the equipment and procedures for sampling the atmosphere in the breathing zone of an individual and for determining the number of fibers accumulated on a filter membrane during the course of an appropriately-selected sampling period. The practice may also be used to sample the atmosphere in a specific location or room of a building (area sampling), where this may be helpful in assessing exposure to workers handling fiber-containing products. 1.4 The ideal working range of this test practice extends from 100 fibers/mm² to 1300 fibers/mm² of filter area. For a 1000-L air sample, this corresponds to a concentration range from approximately 0.04 to 0.5 fiber/mL (or fiber/cm³). Lower and higher ranges of fiber concentration can be measured by reducing or increasing the volume of air collected. However, when this practice is applied to sampling the presence of other, non-asbestos dust, the level of total suspended particulate may impose an upper limit to the volume of air that can be sampled if the filters produced are to be of appropriate fiber loading for fiber counting. 1.5 Users should determine their own limit of detection using the procedure in Practice D 6620. For Reference the NIOSH 7400 method gives the limit of detection as 7 fibers/mm² of filter area. For a 1000 L air sample, this corresponds to a limit of detection of 0.0027 fiber/mL (or fiber/cm³). For OSHA method ID 160 the limit of detection is given as 5.5 fibers/mm² of filter area. For a 1000 L air sample, this corresponds to a limit of detection of 0.0022 fiber/mL (or fiber/cm³). 1.6 If this practice yields a fiber concentration that does not exceed the occupational limit value for the particular regulated fiber variety, no further action may be necessary. If the fiber concentration exceeds the occupational limit value for a specific fiber variety, and there is reason to suspect that the specific fiber variety is mixed with other fibers not covered under the same standard or regulation, the optional method specified in Appendix X1 may be used to measure the concentration or proportion of the fibers counted that are of the regulated variety. 1.7 The mounting medium used in this practice has a refractive index of approximately 1.45. Fibers with refractive indices in the range of 1.4 to 1.5 will exhibit reduced contrast, and may be difficult to detect. 1.8 Fibers less than approximately 0.2 m in diameter will not be detected by this practice. (4) 1.9 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautionary statements, see Section 7.
Testing-equipment; Workplace-monitoring; Analytical-processes; Sampling-methods; Air-sampling; Air-sampling-techniques; Standards; Asbestos-dust; Asbestos-fibers; Air-monitoring; Workplace-monitoring; Author Keywords: Airborne Particulate Matter; Asbestos Fibers; Phase Contrast Microscopy; Sampling; Transmission Electron Microscopy; Workplace Atmospheres
Publication Date
Document Type
Fiscal Year
NTIS Accession No.
NTIS Price
NIOSH Division
Source Name
Standard practice for sampling and counting airborne fibers, including asbestos fibers, in the workplace, by phase contrast microscopy (with and option of transmission electron microscopy).