NIOSHTIC-2 Publications Search
Rapid detection and quantitation of fungal spores from dust samples using real-time PCR.
Keswani-J; Kashon-M; Chen-B
Bioaerosols, fungi, bacteria, mycotoxins and human health: patho-physiology, clinical effects, exposure assessment, prevention and control in indoor environments and work. Johanning E, ed., Albany, NY: Fungal Research Group Foundation, Inc., 2005 Mar; :366-374
Recent advances in real-time PCR have permitted accurate, rapid and quantitative identification of microorganisms in pure cultures regardless of viability or culturability. In this study, a simple sample processing method was investigated for rapid identification and quantitation of fungal spores from dust samples using real-time PCR. The proposed method was evaluated for susceptibility to interference from environmental dust samples. The extent of inhibition was calculated using real-time PCR reactions containing Aspergillus fumigatus spores specific primers and probe and various amounts of dust. No interference (p < 0.05) was detected from 0.2 mg of four real-world dust samples. However, dusts weighing > 0.2 mg compromised the assay. The overall results suggest the potential usefulness of our method for monitoring indoor microbial aerosols containing dusts weighing < / = 0.2 mg using real-time PCR.
Fungi; Fungicides; Dusts; Dust-particles; Dust-sampling; Microorganisms; Aerosols; Aerosol-particles; Aerosol-sampling
Bioaerosols, fungi, bacteria, mycotoxins and human health: patho-physiology, clinical effects, exposure assessment, prevention and control in indoor environments and work