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Aerosolization of microorganisms from metalworking fluids.

Reponen T; Wang HX; Martuzevicius D; Adhikari A; Grinshpun SA; Willeke K
Proceedings of the Health Effects of Mineral Oil Mist and Metalworking Fluids Symposium, October 2-4, 2002, Cincinnati, Ohio. Cincinnati, OH: American Conference of Governmental Industrial Hygienists, 2002 Oct; :12
Workers exposed to metal working fluid (MWF) aerosols may be at risk of developing a variety of respiratory and skin diseases. Microorganisms present as contaminants in MWFs are suspected to be one of the causative agents for respiratory symptoms and diseases in workers. Until now, most of the research has been focused on microorganisms in MWF reservoirs. Limited information is available on the composition and concentration of airborne microorganisms at metalworking sites. The aim of this study is to characterize the aerosolization of different microorganisms from MWFs. First phase of this study included aerosolization of Pseudomonas fluorescence cells, Bacillus subtilis endospores, and Penicillium melinii spores by a Collison nebulizer from three different suspensions: sterilized water, 5%semi-synthetic MWF and 5% soluble oil MWF. The resulting bioaerosols were dried, charge-neutralized and passed into the measurement chamber. The airborne microorganisms in the measurement chamber were sampled by identical Button Personal Inhalable Aerosol Samplers at an airflow rate of 4 liters/min. The concentrations in the nebulizer suspensions and on the Button Sampler filters were analyzed by microscopic enumeration. The microorganism concentration in the suspension was adjusted to 10^7 particles/ml. The concentration of airborne microorganisms ranged from 3xl0/\6 to 2x10^7 particles/m3. The aerosolization ratio the concentration in the air, #/m3 divided by the concentration in the nebulizer suspension, #/mL) varied from 0.4 to 0.7. It was found that the aerosolization of microorganisms does not depend on the type of microorganism or the type of MWF. Future experiments will be performed using a simulator for MWF aerosol generation to investigate the effect of operational parameters (rotation speed, fluid application rate, ventilation rate) on the aerosolization efficiency.
Workers; Occupational-exposure; Aerosols; Aerosol-particles; Microorganisms; Metalworking; Metalworking-fluids; Metalworking-industry; Risk-analysis; Risk-factors; Skin-diseases; Respiratory-system-disorders; Occupational-diseases; Diseases; Air-contamination
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Work Environment and Workforce: Mixed Exposures
Source Name
Proceedings of the Health Effects of Mineral Oil Mist and Metalworking Fluids Symposium, October 2-4, 2002, Cincinnati, Ohio
Performing Organization
University of Cincinnati, Cincinnati, Ohio
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division