An assessment of the developmental toxicity of triethylene glycol (TEG) and triethylene glycol dimethyl ether (TEGDE) in drosophila melanogaster.
Toxicologist 1996 Mar; 30(1)(Part 2):194-195
As part of an ongoing effort to assess the developmental toxicity of selected glycol ethers and their metabolites. Drosophila were exposed throughout development (egg through third instar larva) in culture vials to medium containing equimolar concentrations ( .0013-1.64mM/vial) of triethylene glycol (TEG) and triethylene glycol dimethyl ether (TEGDE). Each vial contained 1 g of powered medium and 5ml of distilled deionized water or a solution of test chemical in water. A mated, untreated, Oregon-R wild-type female was added to each culture vial and allowed to oviposit for 20 hours, then removed. Emerging offspring were collected over 10 days, and examined microscopically (25x) for bent humeral bristles; a morphological defect shown to occur with an increased incidence in flies exposed to developmental toxicants. The incidence of bent bristles was statistically increased (p<0.001) compared to concurrent controls (chi-square) at the two highest TEG concentrations (38/181 at 1.33mM; 29/99 at l.64mM). For TEGDE, the incidence of bent bristles was increased at 0.34mM (10/159; p<0.01) and at 0.66mM (28/171; p<0.001) and 0.99mM (6/25; p<0.001) - no flies emerged at 1.33 and 1.64mM. In contrast to these results, treatment with the documented developmental toxicant 2-methoxyethanol (2ME) resulted in statistically significant increases in bent humeral bristles at concentrations as low as 0.085mM in experiments reported previously using a similar protocol. The results with TEG and TEGDE parallel the developmental toxicity seen in mammals and support further utilization of this Drosophila-based assay as a prescreen for developmental toxicants.
Toxins; Toxic-effects; Glycols; Ethers; Exposure-levels; Exposure-assessment; Microscopic-analysis; Insects
The Toxicologist. Society of Toxicology 35th Annual Meeting, March 10-14,1996, Anaheim, California