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A rapid, sensitive, and specific immunochroma tographic lateral flow assay for anthrax protective antigen immunity status: development and evaluation.

Authors
Sammons-DL; Biaqini-RE; Smith-JP; MacKenzie-BA; Striley-CAF; Robertson-SR; Snawder-JE
Source
2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Holiday Inn Conference Center, Fairborn, Ohio, 2005 Apr; :22
Link
NIOSHTIC No.
20030188
Abstract
Evidence from animals suggests that anti-protective antigen (PA) IgG from vaccination with Anthrax Vaccine Adsorbed (AVA) is protective against B. anthracis infection. Measurement of anti-PA IgG in human sera can be performed using either Enzyme-Linked Immunosorbent Assay (ELISA) or Fluorescent Covalent Microsphere Immunoassay (FCMIA, Clin Lab Diagnostic Immunol 11 :50-55, 2004). Both these methods are laboratory based. We describe the development of a rapid lateral flow immunochromatograhic assay (LFIA) test kit for the measurement of anti-PA IgG in serum or whole blood (50 ul sample) using colloidal gold nanoparticles as the detection reagent and an internal control. Using sera from 19 AVA vaccinees (anti-PA IgG range, 2.4 - 267 ug/ml), 10 controls and PA-supplemented whole blood, we demonstrated the LFIA had a sensitivity of -2 ug/ml anti-PA IgG in sera and -14 ug/ml anti-PA IgG in whole blood. Pre-adsorption of sera with PA yielded negative anti-PA LFIAs. Internal controls were positive for all tests. The diagnostic sensitivity and specificity of the assay (for human sera) were 100% using FCMIA anti-PA IgG as standard. This kit has utility for determining the immune status of individuals (military personnel, decontamination workers) vaccinated with AVA or possibly exposed to sub-clinical levels of B. anthracis.
Keywords
Toxicology; Risk-analysis; Biological-warfare-agents; Biological-weapons; Decontamination; Nanotechnology
Publication Date
20050425
Document Type
Abstract
Funding Type
Interagency Agreement
Fiscal Year
2005
NTIS Accession No.
NTIS Price
NIOSH Division
DART
Source Name
2005 Toxicology and Risk Assessment Conference, Fairborn, Ohio
State
OH
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