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Multiplexed measurement of specific IgGs to five CDC select bioterrorism agents in serum.

Authors
Biaqini-RE; Sammons-DL; Smith-JP; MacKenzie-BA; Striley-CAF; Robertson-SR; Snawder-JE; Quinn-CP
Source
2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Holiday Inn Conference Center, Fairborn, Ohio, 2005 Apr; :21
Link
NIOSHTIC No.
20030187
Abstract
The Centers for Disease Control and Prevention has classified several agents which may be used in a bioterrorism attack as "select agents". They are classified as such based on ease of dissemination, mortality/morbidity rate, and potential for social disruption. A subset of these agents include Bacillus anthracis (Ba), Yersinia pestis (Yp), Francisella tularensis (Ft) , Ricin toxin (RT) and Staphylococcal enterotoxin B (SEB). Exposure/infection with these agents has been shown to cause the production of specific serum IgG antibodies. Comparison of pre- and post-incident IgG antibody levels has been shown to be a useful method to biologically monitor decontamination and clean-up workers for potential exposure to bioterrorism agents (Occ Environ Med 61: 703-708, 2004). We describe a fluorescent covalent microsphere immunoassay (FCMIA) to measure specific IgG antibodies to Ba (protective antigen [PA] and lethal factor [LF]), Yp (F1 and V antigens), Ft, RT and SEB simultaneously in human Ba vaccinee sera which has been fortified with animal select agent specific IgG antibodies. Intra- and interassay coefficients of variation were <15% (N=3). There were no significant differences (P>0.70) in dilution curves when the assays were performed individually vs. multiplexed. When the observed vs. expected dilution curves were compared, highly linear relationships were observed (mean r2=0.995 + 0.006 [SO]). Finally, the curves yielded linear responses for most analytes upon serum dilution from 1 x102 to 1 x105. These data indicate that multiplexed FCMIA is a method to measure specific IgGs in serum to CDC select agents and may be of value in screening either decontamination workers or the general population for exposures/infections to these agents.
Keywords
Toxicology; Risk-analysis; Biological-warfare-agents; Biological-weapons; Decontamination
Publication Date
20050425
Document Type
Abstract
Funding Type
Interagency Agreement
Fiscal Year
2005
NTIS Accession No.
NTIS Price
NIOSH Division
DART
Source Name
2005 Toxicology and Risk Assessment Conference
State
GA; OH
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